Alternative pre-mRNA Splicing

Alternative pre-mRNA Splicing

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This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing in twelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative pre-mRNA splicing in vitro and in vivo, Manipulation of splicing events, and Bioinformatic analysis of alternative splicing. A theoretical introduction and practical guide for molecular biologists, geneticists, clinicians and every researcher interested in alternative splicing. Website: 50 end ofthe intron, recognized initially by U1 snRNP, is identified by the 9 nt consensus sequence shown. A strong base-pairing between U1 snRNA and the 50 splice site increases the likelihood that a splice site is used; (b) Diagram ofthe 30 splice site. ... the spliceosomal protein U2AF binds preferentially to pyrimidines, the longer the stretch of uninterrupted pyrimidines in the PPT, the more likelyanbsp;...

Title:Alternative pre-mRNA Splicing
Author: Stefan Stamm, Chris Smith, Reinhard Lührmann
Publisher:John Wiley & Sons - 2012-08-01

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